Fig. 1: FHL1 expression is markedly decreased in endometrial cells of RIF patients.
From: FHL1 mediates HOXA10 deacetylation via SIRT2 to enhance blastocyst-epithelial adhesion
A GEO database data (GSE103465) show that FHL1 is expressed in the endometria of women with RIF and fertile controls. B The relative expression of FHL1 mRNA in RIF (n = 18) and CTR (fertile women, n = 14) human endometrial tissues was measured by qRT–PCR. The 18S gene was used as a loading control. **P < 0.01, by Student’s t test. C Western blot analysis to detect FHL1 in RIF (n = 24) and CTR (fertile woman, n = 22) human endometrial tissues. Normalization was performed with the GAPDH protein as a housekeeping protein. ***P < 0.001, by Student’s t test. D, E The localization of FHL1 expression in RIF (n = 3) and CTR (fertile woman, n = 3) human endometria was detected by immunohistochemistry. The total expression of FHL1 in RIF and CTR endometria and its expression in epithelial cells were analyzed. IgG was used as a negative control. GE: gland, S: stroma. *P < 0.05, by Student’s t test. Scale bar = 50 μm.
