Fig. 4: FHL1 is a newly identified HOXA10-interacting molecule.
From: FHL1 mediates HOXA10 deacetylation via SIRT2 to enhance blastocyst-epithelial adhesion
A Bioinformatics-based prediction of structural interactions of FHL1 with HOXA10 protein. B, C Anti-Myc/Flag immunoprecipitation followed by immunoblotting analysis of whole-cell lysates of HEK293T cells transfected with Flag-FHL1 either alone or together with Myc-HOXA10. D Ishikawa whole-cell lysates were subjected to anti-HOXA10 immunoprecipitation followed by immunoblotting analysis. Goat IgG was used as a negative control. E Schematic diagram of the HOXA10 mutants in Fig. 4F. F Flag-FHL1 and different Myc-HOXA10 mutants were cotransfected in HEK293T cells, and whole-cell lysates were collected for anti-Flag immunoprecipitation and immunoblotting analysis. G Endogenous FHL1 and HOXA10 significantly colocalized in Ishikawa nuclei.
