Fig. 4: M2-like macrophages express higher levels of Slc7a11 than non-M2-like macrophages.

RNA-seq was conducted for four different types of Ly6C^low macrophages; uIRI kidney cortex, sham kidney cortex, uIRI-Can, and Sham-Can. (n = 3 per each kidney sample, n = 4 per each tumor sample). a Principal component analysis (PCA) shows different expression patterns between uIRI-treated samples and Sham-treated samples among Ly6C^low macrophages in the kidney (left) and Ly6C^low macrophages in cancer (right); blue color represents uIRI-treated samples, green color represents Sham-treated samples. b, c Heatmap shows differentially expressed genes. b 441 genes were upregulated and 306 genes were downregulated in Ly6C^low macrophages sorted from the uIRI kidney cortex compared to those from the sham kidney cortex with FDR < 0.001 and log fold change (log FC) more than 2. c 93 genes were upregulated and 59 genes were downregulated in Ly6C^low macrophages sorted from uIRI-Can compared to those from Sham-Can with FDR < 0.05. Representative gene tracks of RNA-seq signals around Ccl22 in kidney samples (d), and Spp1 in tumor samples (e). RNA-seq signals are visualized using Integrative Genomics Viewer (Version 2.11.3) (http://software.broadinstitute.org/software/igv/). f 26 genes were commonly upregulated in Ly6C^low macrophages sorted from the uIRI kidney cortex and uIRI-Can. g Top 20 Gene Ontology (GO) terms enriched among upregulated genes in uIRI kidney included the response to oxidative stress. h Scatter plots show the locus of Slc7a11 among differentially expressed genes in kidney sample (left), and in tumor sample (right). i In qPCR, Slc7a11 expression was upregulated in F4/80^lowLy6C^low macrophages compared to F4/80^highLy6C^low macrophages both in uIRI kidney cortex (left) and uIRI-Can (right). (n = 3 per each F4/80^highLy6C^low macrophage group, n = 4 per each F4/80^lowLy6C^low macrophage group; two-sided Mann–Whitney U test). uIRI unilateral ischemia-reperfusion injury, uIRI-Can kidney cancer inoculated into the kidney subcapsule after unilateral IRI, Sham-Can kidney cancer inoculated into the kidney subcapsule after sham operation.