Fig. 2: Loss of cilia in Myo5a-deficient cells increases susceptibility to necroptotic death. | Cell Death Discovery

Fig. 2: Loss of cilia in Myo5a-deficient cells increases susceptibility to necroptotic death.

From: Primary cilia suppress Ripk3-mediated necroptosis

Fig. 2

A Neutral-red assay in control and Myo5a−/− cells after RCD induction through TNFα (TNF, 4 ng/100 µl) and cycloheximide (CHX, 2 µg/100 µl) for 16 h. Additionally, caspase-8 inhibitor emricasan (Em, 10 µM) and necroptosis inhibitor necrostatin-1s (Nec1s, 40 µM) were used for 16 h (n = 4). B Immunoblot analysis of ciliated and non-ciliated cells using the apoptosis marker cleaved caspase 3 (~17 kDa) and the necroptosis marker phospho-Mlkl (~56 kDa). As housekeeping control either pan-actin (~30 kDa) or beta-tubulin (~55 kDa) were used (n = 3). C Live-cell imaging over the period of 24 h after treatment with TNF and CHX (TC), and TNF, CHX, and Em (TCE) or DMSO as control. Cells were stained with the dead cell marker DiYO-1. Images were captured every 2 h (N = 8).

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