Fig. 4: The inhibition of RIPK3 suppresses cell death and improved cell viability after high-dose UVA irradiation. | Cell Death Discovery

Fig. 4: The inhibition of RIPK3 suppresses cell death and improved cell viability after high-dose UVA irradiation.

From: UVA induces retinal photoreceptor cell death via receptor interacting protein 3 kinase mediated necroptosis

Fig. 4

A LDH release analysis of 661w cells treated with Endaravone (100 μM), Nec-1 (15 μM), Z-VAD (15 μM), combination of Nec-1 (15 μM) and Z-VAD (15 μM), GSK 872 (2 μM) after 10.5 J/cm2 UVA irradiation (n = 6 per group). B Cell viability analysis of 661w cells treated with Endaravone (100 μM), Nec-1 (15 μM), Z-VAD (15 μM), combination of Nec-1 (15 μM) and Z-VAD (15 μM), GSK 872 (2 μM) after 10.5 J/cm2 UVA irradiation by MTT assay (n = 6 per group). C Representative images of annexin V (green) and PI (red) staining in cells treated with GSK 872 (Scale Bar: 20 μm) (n = 6 per group). D Analysis of cell death types in cells treated with GSK 872 (n = 6 per group). E Analysis of late PCD cells in cells treated with GSK 872 (n = 6 per group). F ROS production analysis by testing the amount of protein carbonyl in cells treated with GSK 872 after 10.5 J/cm2 UVA irradiation (n = 6 per group). Statistical significance was analyzed with the unpaired Student’s t-test. Statistical significance of compared with 0 J/cm2, 3.5 J/cm2, 7 J/cm2 is indicated as *, # and &. *P, #P or &P < 0.05, **P, ##P or &&P < 0.01, ***P, ###P or &&&P < 0.001, ****P, ####P or &&&&P < 0.0001. All values are expressed as mean ± SD.

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