Fig. 2: The inhibitory effect of CDO1 on the proliferation in GC cells. | Cell Death Discovery

Fig. 2: The inhibitory effect of CDO1 on the proliferation in GC cells.

From: Cysteine dioxygenase 1 attenuates the proliferation via inducing oxidative stress and integrated stress response in gastric cancer cells

Fig. 2

A The re-expression efficiency of CDO1 in MKN45 (left panel) and NCI-N87 (right panel) cells was verified by immunoblots. B Cellular growth of MKN45 (left panel) or NCI-N87 (right panel) cells from N.C. or CDO1-restored group was assayed using CCK-8 at the indicated time points respectively (N = 6, mean ± SD), showing that CDO1 remarkably suppressed the proliferation in these two GC cell lines. C MKN45 (left panel) or NCI-N87 (right panel) cells from N.C. or CDO1-restored group were stained with EdU and analyzed flow cytometry, showing that CDO1 markedly blocked the proliferation in GC cells. One representative result (N = 3, mean ± SD) are graphically presented here. D, E Tumor xenografts model based on female Balb/c nude mice demonstrated that the proliferation of MKN45 (D) and NCI-N87 (E) cells was substantially inhibited by CDO1 in vivo, as evidenced by the delayed growth at the indicated time intervals and the decreased weights of the tumor masses from MKN45 or NCI-N87 cells. **p < 0.01, ***p < 0.001.

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