Fig. 1: Bcl-2 prevents classical characteristics associated with dex-induced apoptosis in S49 T cells. | Cell Death Discovery

Fig. 1: Bcl-2 prevents classical characteristics associated with dex-induced apoptosis in S49 T cells.

From: Overcoming apoptotic resistance afforded by Bcl-2 in lymphoid tumor cells: a critical role for dexamethasone

Fig. 1

S49 (Neo) cells treated with 2.5 × 10−7 M dex for 48 h resulted in the loss of membrane integrity as determined by the increase in the number of cells which were stained with the vital dye, propidium iodide (PI), an increase in the number of cells which had a decrease in their ability to scatter light in the forward direction, indicating a population of cells with decreased cell size, depolarization of the mitochondrial membrane potential (MMP), evident by the increase in JC-1 monomers and decrease in JC-1 aggregates, and the presence of a sub-diploid peak of DNA, indicative of degraded DNA. In contrast, S49 (Bcl-2) cells treated with dex under identical conditions did not result in any significant changes in membrane integrity or cell size. Interesting, mitochondrial membrane hyperpolarization was observed along with a block at the G1/S phase of the cell cycle. Data represent the mean (+/− SEM) of 3-5 independent experiments.

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