Fig. 1: The fusion of cortical and blood vessel organoids. | Cell Death Discovery

Fig. 1: The fusion of cortical and blood vessel organoids.

From: Cortical-blood vessel assembloids exhibit Alzheimer’s disease phenotypes by activating glia after SARS-CoV-2 infection

Fig. 1

A Schematice of the method of the fusion of blood vessel organoids (BVOs) and cortical organoids (COs). B bright-field images of fused cortical-blood vessel organoids (fCBOs) during initial 3 days post fusion. Scale bars = 1 mm. C Bright-field images of COs and fCBOs on days 29, 43, 57 and 71 of cortical differentiation. Scale bars = 1 mm. D Quantification of the diameter during the 71 days of cortical differentiation (n = 10). E Whole-mount staining of fCBOs for endothelial marker CD31 and neuronal marker TUJ1 on day 43. Newly generated neurites (arrow). Scale bar = 100 μm. F Left, immunostaining of fCBOs for CD31 and TUJ1 (day 57). Penetrated blood vessels in cortical organoid region (yellow box), sprouted neurites to blood vessel organoid region (organge box). Scale bar = 100 μm. G Immunostaining of COs and fCBOs for pericyte marker PDGFRβ on day 57. Scale bar = 100 μm. Values represent mean with individual data points plotted. Error bars are SEM of the mean. *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired two-sided t-test.

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