Fig. 3: SARS-CoV-2 infects fused cortical-blood vessel organoids. | Cell Death Discovery

Fig. 3: SARS-CoV-2 infects fused cortical-blood vessel organoids.

From: Cortical-blood vessel assembloids exhibit Alzheimer’s disease phenotypes by activating glia after SARS-CoV-2 infection

Fig. 3

A Relative viral RNA levels for SARS-CoV-2 N1 and N2 gene of SARS-CoV-2 infected COs and fCBOs normalized to mock controls by qRT-PCR (n = 6). B Immunostaining of SARS-CoV-2 infected fCBOs for SARS-CoV-2 nucleocapside protein (NP) and ACE2 on day 57. Cortical organoid region (left) and blood vessel organoid region (right). Scale bars = 50 μm. C Immunostaining of SARS-CoV-2 infected COs and fCBOs for NP in ventricular zone (yellow box) and superficial layer (orange box). Scale bar = 100 μm. D Immunostaining of SARS-CoV-2 or mock infected COs and fCBOs for TUNEL and NP. Scale bars = 100 μm. E The percentage of NP- or NP+TUNEL+ cells in DAPI+ cells of SARS-CoV-2 or mock infected COs and fCBOs (n = 5 organoids with 4 images per organoid). F Immunostaining of SARS-CoV-2 or mock infected fCBOs for NP and hypoxia marker HIF1α. Scale bars = 100 μm. G The percentage of HIF1α+ cells in NP- (red boxes) or NP+ (yellow boxes) area of SARS-CoV-2 infected fCBOs (n = 5 fCBOs, 4 areas per organoid). H Immunostaining of SARS-CoV-2 or mock infected fCBOs for CD31 and activated endothelial marker ICAM1. Scale bars = 100 μm. Values represent mean with individual data points plotted. Error bars are SEM of the mean. *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired two-sided t-test.

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