Fig. 6: NSUN2 promotes Nrf2 mRNA m5C methylation modification in H9C2 cells.
From: NSUN2 alleviates doxorubicin-induced myocardial injury through Nrf2-mediated antioxidant stress
A IF detected the intracellular m5C level of H9C2 cells after DOX treatment (n = 3, Bar = 20 μm). B Dot blot detected the changes of m5C methylation in H9C2 cells after DOX treatment. C IF detected the effect of NSUN2 overexpression on NSUN2 and Nrf2 expression levels and m5C enrichment in H9C2 cells (Bar = 20 μm). D m5C MeRIP-PCR detected the effect of NSUN2 overexpression on the enrichment of m5C in H9C2 cells (n = 4). E RT-PCR detected the effect of NSUN2 overexpression on the half-life of Nrf2 mRNA (n = 3). F m5C MeRIP test the content of m5C modified Nrf2 mRNA in NSUN2 and GFP group. G RT-PCR detected the effect of NSUN2 overexpression on the half-life of Nrf2 mRNA when treated with methylase inhibitor 3-DZA (n = 3). The measurement data was showed as mean ± standard deviation, and compared by student’s t-test. **P < 0.05; **P < 0.01; ***P < 0.001.
