Fig. 4: Suppression of ESCC cell proliferation and migration by circTRPS1-2. | Cell Death Discovery

Fig. 4: Suppression of ESCC cell proliferation and migration by circTRPS1-2.

From: Circular RNA circTRPS1-2 inhibits the proliferation and migration of esophageal squamous cell carcinoma by reducing the production of ribosomes

Fig. 4: Suppression of ESCC cell proliferation and migration by circTRPS1-2.The alternative text for this image may have been generated using AI.

a, b Confirmation of circTRPS1-2 overexpression or knockdown in ESCC cells, based on qRT–PCR. Expression of linear TRPS1 was not affected. β-actin was the reference gene. c, d Colony formation assay to assess the survival of ESCC cells over- or underexpressing circTRPS1-2. e, f EdU incorporation assay to evaluate the proliferation of ESCC cells. Magnification, 200×. Scale bar, 100 µm. g Cell counting Kit-8 assay to evaluate cell viability. h, i Wound healing assay to evaluate the migration of ESCC cells. Magnification, 100×. Scale bar, 200 µm. j, k Transwell assay to measure ESCC cell migration. Magnification, 100×. Scale bar, 200 µm. Data are mean ± SD (n = 3 independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001. circTRPS1-2, circTRPS1-2 overexpression plasmid; mock, negative control plasmid; si-NC, negative control siRNA; si-circ#1 or 2, circTRPS1-2 siRNA#1 or 2, respectively.

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