Fig. 6: Binding of circTRPS1-2 to ribosomal proteins and effects on ribosome biogenesis. | Cell Death Discovery

Fig. 6: Binding of circTRPS1-2 to ribosomal proteins and effects on ribosome biogenesis.

From: Circular RNA circTRPS1-2 inhibits the proliferation and migration of esophageal squamous cell carcinoma by reducing the production of ribosomes

Fig. 6: Binding of circTRPS1-2 to ribosomal proteins and effects on ribosome biogenesis.The alternative text for this image may have been generated using AI.

a Circos overlap plot showing the number of proteins binding to the sense and antisense probes in the circTRPS1-2 pulldown assay in K150 cells. b GO enrichment analysis, Reactome pathway analysis and KEGG pathway enrichment analysis of proteins binding specifically to circTRPS1-2. c Interactions among the proteins in panel b. d Western blotting against several ribosomal proteins in the RNA precipitate. e RIP was performed in K150 cell lysates using antibodies against RPS4X, RPS8, RPS24, RPL7, RPL11 or IgG. Enrichment of circTRPS1-2 was detected by qRT–PCR, relative to circTRPS1-2 expression of input group. Results are from three independent experiments. f Immunofluorescence of K150 cells to investigate the subcellular localization of RPS4X, RPS8, RPS24, RPL7 and RPL11. Magnification, 400×. Scale bar, 100 µm. g Absorbance (260 nm) of total ribosome extracts from same number of K150 cells over- or underexpressing circTRPS1-2. Results are from three independent experiments. h Representative electron micrographs of ribosomes in K150 cells over- or underexpressing circTRPS1-2. The small and black particles in the cytoplasm are ribosomes. Magnification, 20000×. Scale bar (upper left of images), 0.3 µm. Results are from three independent experiments. Data are mean ± SD. *P < 0.05, **P < 0.01. circTRPS1-2, circTRPS1-2 overexpression lentivirus; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; mock and sh-NC, negative control lentivirus; RIP, RNA immunoprecipitation; RPS4X, ribosomal protein S4 X-linked; RPS8, ribosomal protein S8; RPS24, ribosomal protein S24; RPL7, ribosomal protein L7; RPL11, ribosomal protein L11; sh-circ, circTRPS1-2 shRNA interference lentivirus.

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