Fig. 5: CFI-400945 induces apoptosis in vascular SMCs. | Cell Death Discovery

Fig. 5: CFI-400945 induces apoptosis in vascular SMCs.

From: Polo-like kinase 4 inhibitor CFI-400945 inhibits carotid arterial neointima formation but increases atherosclerosis

Fig. 5

SMCs were treated with DMSO or different concentrations of CFI-400945 for 72 h. A–B Cells were then fixed with 4% paraformaldehyde for the TUNEL assay. Negative control: cells were incubated with the labeling solution instead of the TUNEL reaction solution. Positive control: cells were treated with recombinant DNase I for 10 min to induce DNA strand breaks before labeling. A Representative LSC scattergrams (upper) and histograms (lower). B Cumulative data showing the percentages of TUNEL-positive cells (one-way ANOVA with Tukey’s post hoc; n = 5). C Representative WB. After treatment with and without CFI-400945 (1 μM) for different times, SMCs were harvested for WB analysis of caspase 3, 7, and 9 and their cleaved forms.

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