Fig. 6: CFI-400945 causes SMC polyploidization, leading to apoptosis. | Cell Death Discovery

Fig. 6: CFI-400945 causes SMC polyploidization, leading to apoptosis.

From: Polo-like kinase 4 inhibitor CFI-400945 inhibits carotid arterial neointima formation but increases atherosclerosis

Fig. 6

Vascular SMCs were pre-treated with hydroxyurea (HU, 100 μg/ml) or pan-caspase inhibitor (PCI) Z-VAD-FMK (25 μM) for 1 h, followed by CFI-400945 treatment for 24 h. A Representative LSC scattergrams of cells fixed for BrdU staining. B Cumulative data showing the distribution of cells with ≥4 N DNA. C, Representative LSC scattergrams of cells fixed for cleaved caspase 3 staining. D Cumulative data showing the percentage of cleaved caspase 3-positive cells. E Representative LSC scattergrams of cells fixed for cleaved caspase 3 staining. F Cumulative data showing the percentage of cleaved caspase 3-positive cells. Data were analyzed by one-way ANOVA with Tukey’s post hoc; n = 3–5. ***p < 0.001.

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