Fig. 2: Th1 cells inhibit the growth of tumor cells for malignant ascites by secreting IFN-γ. | Cell Death Discovery

Fig. 2: Th1 cells inhibit the growth of tumor cells for malignant ascites by secreting IFN-γ.

From: Interferon-γ secreted by recruited Th1 cells in peritoneal cavity inhibits the formation of malignant ascites

Fig. 2

A Flow cytometric analysis revealed that Th1 cells induced apoptosis of H22 cells and S180 cells in vitro after 48-h incubation with Th1 cells. Upper panel: representative flow cytometric dot plots of apoptosis assay (left) and the percentages of apoptotic cells (right) revealing Th1 cells induced apoptosis of H22 cells in vitro; lower panel: representative flow cytometric dot plots of apoptosis assay (left) and the percentages of apoptotic cells (right) revealing Th1 cells induced apoptosis of S180 cells in vitro. The difference between two groups was determined by t test. B proliferative activity assay showing Th1 cells suppressed the proliferation of H22 cells (upper) and S180 cells (lower). The difference between two groups was determined by t test. C Representative dot plots of flow cytometric analysis showing the percentage of CD4+ cells in IFN-γ-producing cells in a patient with malignant ascites (left); the percentage of CD4+ cells in IFN-γ-producing cells in patients with malignant ascites (right); D Representative dot plots of flow cytometric analysis showing the percentage of CD4+ cells in IFN-γ-producing cells in a rat with malignant ascites (left); the percentage of CD4+ cells in IFN-γ-producing cells in rats with malignant ascites (right). E Flow cytometric analysis revealed that IFN-γ induced apoptosis of H22 cells and S180 cells in vitro after 48-h incubation with IFN-γ. Upper panel: representative flow cytometric dot plots of apoptosis (left) and the percentage of apoptotic cells (right) revealing IFN-γ induced the apoptosis of H22 cells in vitro; lower panel: representative flow cytometric dot plots of apoptosis assay (left) and the percentage of apoptotic cells (right) revealing IFN-γ induced the apoptosis of S180 cells in vitro. The difference between two groups was determined by t test; F proliferative activity assay showing IFN-γ suppressed the proliferation of H22 cells (upper) and S180 cells (lower). The difference between two groups was determined by t test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

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