Fig. 2: Inactivation of GSK3β blocks apoptosis induction of FLT3-ITD inhibitors. | Cell Death Discovery

Fig. 2: Inactivation of GSK3β blocks apoptosis induction of FLT3-ITD inhibitors.

From: The GSK3β/Mcl-1 axis is regulated by both FLT3-ITD and Axl and determines the apoptosis induction abilities of FLT3-ITD inhibitors

Fig. 2

A MOLM-13 cells were pretreated with 100 ng/mL TPA and then cotreated with each inhibitor at 50 nM for 24 h. Apoptotic cells were examined by Annexin V/PI staining. B Western blot analysis of protein changes in TPA-pretreated MOLM-13 cells followed by each inhibitor. C MOLM-13 cells pretreated with GSK3β inhibitor CHIR-99021 and SB216763 for 4 h and then with FLT3-ITD inhibitors for 24 h. Apoptotic cells were detected by AO-EB staining. D Protein regulation of MOLM-13 cells treated with SB216763 and FLT3-ITD inhibitors for 24 h. E MOLM-13 cells were treated with 5 μM CHIR-99021 and quizartinib for 24 h. Apoptotic cells were detected by Annexin V/PI staining. F Protein regulation of MOLM-13 cells treated with CHIR-99021 and quizartinib for 24 h. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the FLT3-ITD inhibitor group.

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