Fig. 3: Transcriptional regulation network captured by chromatin interactions in cardiomyocytes.
From: Dynamic chromatin landscape encodes programs for perinatal transition of cardiomyocytes
A Experimental strategy to capture chromatin interactions in perinatal cardiomyocytes. B Proportion of the interaction types. C Histogram of promoters with different numbers of interactions in HiChIP promoter-involving interactions. D Histogram of promoter-involving interactions with different sizes. E Genome browser view at Nexn-AE1 locus with chromatin accessibility and chromatin interaction in cardiomyocytes. The AE1 and Nexn promoter are marked with blue and orange boxes, respectively. The interaction between AE1 and Nexn promoter is indicated by the brown arch (left). LacZ staining in mouse embryo from VISTA database reveals heart enhancer activity of AE1 (right). F Gene expression of promoter-associated genes and the nearest or the interacting genes of the same non-promoter regulatory element (center line, median; box limits, upper and lower quartiles, Wilcoxon rank sum test, P for non-promoter nearest gene vs. interacted gene expression in E18.5, P1, P3, and P7 are 2.2E-16, 1.484E-14, 1.388E-14 and 2.511E-14, respectively. * indicates P < 0.05). G Top 10 tissues with enriched expression of target genes of the distal regulatory elements.
