Fig. 5: Dynamic regulatory program involves transcription factor MEF2 and AP1.

A Motif enrichment on downregulated elements and upregulated elements in cardiomyocytes transcriptional network. B and C Network diagram of top 15 gene ontology and pathway terms associated with the genes regulated by MEF2 (B) or AP1 (C). Each node set represents a term and each node in the set represents a gene. The colors of node sets represent the term category. D Co-staining of cTnT and DAPI staining of NRVCs treated with TSA/si-Mef2a (–/–, +/–, –/+, +/+). Scale bars, 100 μm. The representative image is shown. E Quantifications of cell area (Wilcoxon rank sum test, P for TSA/si-Mef2a (–/–) vs. (+/–) and (+/–) vs. (+/+) are <2.2E-16, <2.2E-16, respectively. * indicates P < 0.05). F Co-staining of α-actinin and Aurora B of NRVCs treated with scramble siRNA or si-Junb. Scale bars, 100 μm. The representative image from one of three independent experiments is shown. G Percentage of Aurora B⁺ NRVCs (n = 3, two-tailed Student’s t-test, P = 0.044, * indicates P < 0.05). H Co-staining of α-actinin and DAPI staining of NRVCs treated with scramble siRNA or si-Junb. Scale bars, 100 μm. The representative image from one of three independent experiments is shown. I Percentage of binucleated NRVCs (n = 3, two-tailed Student’s t-test, P = 0.0063, * indicates P < 0.05).