Fig. 4: Conjugated BAs induced TNFRSF12A expression and pyroptosis in hepatocytes. | Cell Death Discovery

Fig. 4: Conjugated BAs induced TNFRSF12A expression and pyroptosis in hepatocytes.

From: Hepatic TNFRSF12A promotes bile acid-induced hepatocyte pyroptosis through NFκB/Caspase-1/GSDMD signaling in cholestasis

Fig. 4: Conjugated BAs induced TNFRSF12A expression and pyroptosis in hepatocytes.The alternative text for this image may have been generated using AI.

A TNFRSF12A mRNA transcripts, and (B) protein levels in PLC/RPF/5-ASBT cells treated with 100 μM TCA, TCDCA, GCA, GCDCA, or TCDCA. *p < 0.05 compared to control (DMSO); Time-dependent effects of TCA on TNFRSF12A (C) mRNA and (D) protein expression in PLC/RPF/5-ASBT cells; (E) Western blot analysis of effects of conjugated BAs (TCA, GCA, GCDCA, TDCAC, or TCDCA, 100 μM) on protein expression of pro-Caspase-1, cleaved-Caspase-1, GSDMD, cleaved-GSDMD, caspase-3, Bcl2, and Bax in PLC/RPF/5-ASBT cells; F Representative images of TEM showing features of pyroptosis, including membrane pores, cell swelling, and large bubbles blowing from the plasma membrane in PLC/RPF/5-ASBT cells treated with 100 μM TCA (scale bar: 5 µm).

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