Fig. 6: Conjugated BAs stimulated Cxcl2 and Ccl2-mediated macrophage infiltration and subsequent TWEAK production to further enhance TNFRSF12A-induced hepatocyte pyroptosis. | Cell Death Discovery

Fig. 6: Conjugated BAs stimulated Cxcl2 and Ccl2-mediated macrophage infiltration and subsequent TWEAK production to further enhance TNFRSF12A-induced hepatocyte pyroptosis.

From: Hepatic TNFRSF12A promotes bile acid-induced hepatocyte pyroptosis through NFκB/Caspase-1/GSDMD signaling in cholestasis

Fig. 6

A Serum TWEAK levels in control patients (n = 20) and OC patients (n = 34). *p < 0.05 vs. control patients; B The levels of TWEAK mRNA transcripts in PLC/RPF/5-ASBT cells or primary mouse hepatocytes treated with 100 μM TCA, GCA, GCDCA, TDCAC, and TCDCA; (C) Immunofluorescence labeling of F4/80 (a specific marker for macrophages) in a human control liver and an OC liver; D The levels of TWEAK mRNA transcripts in THP1 cells treated with 100 μM GCA. *p < 0.05 vs. DMSO control group; E A diagram for transwell assays (left) and migration ability of co-cultured THP1-derived macrophages (right) under conjugated BA stimulation; F The TWEAK levels in cell supernatant of co-cultured THP-derived macrophages and primary mouse hepatocytes following treatment with DMSO as control, GCA or TCDCA; G The levels of Cxcl2, Ccl2, IL-18, IL-1β, and Tweak mRNA transcripts in co-cultured primary mouse hepatocytes following treatment with DMSO as control, GCA or TCDCA. *p < 0.05 vs. DMSO control group; H The levels of Tnfrsf12a, Nlrp3, cleaved-Caspase-1, cleaved-GSDMD, and cleaved- IL-1β in primary mouse hepatocytes treated with DMSO as control, GCA, and/or TWEAK. OC, obstructive cholestasis.

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