Fig. 1: miR-187 targets the 5′-UTR of the sow TGFBR2 gene.
From: A regulatory network controlling ovarian granulosa cell death
A Schematic diagram of TGFBR2 5′ regulatory region. B Sequence alignment of TGFBR2 5′-UTR from Duroc and Yorkshire pigs. RBP-binding elements are indicated by underlining. RNA G-quadruplexes are indicated by blue boxes. C The MRE motif of miR-187 in TGFBR2 5′-UTR. D Prediction of minimum free energy (MFE) of binding miR-187 and TGFBR2 5’-UTR using RNAhybrid. E Schematic showing the reporter vectors of TGFBR2 5’-UTR containing wild-type and mutated MRE of miR-187. F Luciferase reporter assays. G Subcellular distribution of miR-187 in sow GCs. Nuclear and cytoplasmic RNAs were isolated from GCs, and levels of miR-187 and internal reference genes (U6 in the nucleus and GAPDH in the cytoplasm) were detected using qPCR. H RNA pull-down assay. TGFBR2 5′-UTR were pulled down using biotin-labeled probes of miR-187 in GCs and detected by qPCR. Data represent means ± SEM for three independent experiments. *p < 0.05.
