Fig. 2: miR-187 maintains TGFBR2 levels in GCs by influencing their stability.
From: A regulatory network controlling ovarian granulosa cell death
A–C After incubating GCs with miR-187 mimics, levels of miR-187 (A), TGFRB2 mRNA (B), and TGFBR2 protein (C) were measured. D–F After incubating GCs with miR-187 inhibitor, levels of miR-187 (D), TGFRB2 mRNA (E), and TGFBR2 protein (F) were measured. G, H mRNA stability assays. G After transfection of miR-187 mimics or mimics NC into GCs for 10 h, ActD was added, then TGFBR2 mRNA levels were detected at 0, 2, 4, 6, 8, and 10 h. H After co-transfection with the plasmid pGL3-CMV-MREwt and miR-187 mimics or mimics NC into KGN cells for 10 h, ActD was added, then firefly luciferase gene mRNA levels were detected at 0, 2, 4, 6, 8, and 10 h. Data are represented as means ± SEM for three independent experiments. *p < 0.05; **p < 0.01.
