Fig. 3: miR-187 regulates TGF-β signaling and GC apoptosis by targeting TGFBR2. | Cell Death Discovery

Fig. 3: miR-187 regulates TGF-β signaling and GC apoptosis by targeting TGFBR2.

From: A regulatory network controlling ovarian granulosa cell death

Fig. 3

A, B miR-187 activates TGF-β signaling in GCs. GCs were transfected with miR-187 mimics (A) or inhibitor (B), and phosphorylated SMAD3 (p-SMAD3) and total SMAD3 (t-SMAD3) protein levels were detected using western blot. C, D FACS detection of apoptosis rate in GCs after co-treatment of miR-187 mimics and TGFBR2-siRNA (C) or miR-187 inhibitor and pcDNA3.1-TGFBR2 (D). Data are represented as means ± SEM for three independent experiments. *p < 0.05; **p < 0.01.

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