Fig. 4: NORHA regulates TGF-β signaling by adsorbing miR-187.
From: A regulatory network controlling ovarian granulosa cell death
A Schematic of reporter vectors expressing NORHA containing wild-type or mutated MREs of miR-187. B Luciferase reactivity was determined and normalized to the NC group. C RNA pull-down assay. miR-187 was pulled down using biotin-labeled probes for NORHA containing MRE4 motif in GCs and then detected using qPCR. D, E GCs were treated with plasmid pcDNA3.1-NORHA, TGFBR2 mRNA levels were detected using qPCR (D), and protein levels were detected using western blotting (E). F TGFBR2 protein levels in GCs after co-treatment of pcDNA3.1-NORHA and miR-187 mimics were detected by western blot. Data are represented as means ± SEM for three independent experiments. *p < 0.05; **p < 0.01.
