Fig. 4: TRIM3 could alter downstream pathway of p53 and MDR1 expression by retaining p53 in the cytoplasm to decrease its nuclear expression. | Cell Death Discovery

Fig. 4: TRIM3 could alter downstream pathway of p53 and MDR1 expression by retaining p53 in the cytoplasm to decrease its nuclear expression.

From: Dual roles of TRIM3 in colorectal cancer by retaining p53 in the cytoplasm to decrease its nuclear expression

Fig. 4: TRIM3 could alter downstream pathway of p53 and MDR1 expression by retaining p53 in the cytoplasm to decrease its nuclear expression.

a The target genes and downstream pathway of TRIM3 and p53 predicted by IPA software. TRIM3 and p53 were used as seeds to construct their related interaction network available in the Ingenuity database. Solid lines: direct interaction; Solid lines without arrow head: molecular interaction; Solid lines with arrow head: molecular regulation; b1 The expression of p53 in the nuclei and cytoplasm after TRIM3 overexpression; b2 The quantification of p53 determined by densitometry of protein bands. GAPDH was the loading control in the nuclei and total; Histone H3 was the loading control in the cytoplasm; c The expression of downstream pathway of p53 after TRIM3 overexpression in the three p53-specific cell lines (HCT116+/+, HCT116−/− and SW480); d The survival curves of CRC patients with regard to TRIM3 expression and different chemotherapy regiments; e The expression of downstream pathway of TRIM3 in wtp53 and mutp53 cells after treatment with oxaliplatin.

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