Fig. 6: Sox8 mediated the autophagy induced by MSU evaluated by Western blot assay. | Cell Death Discovery

Fig. 6: Sox8 mediated the autophagy induced by MSU evaluated by Western blot assay.

From: Downregulation of Sox8 mediates monosodium urate crystal-induced autophagic impairment of cartilage in gout arthritis

Fig. 6

Overexpression of Sox8 inhibited the autophagy induced by MSU evaluated by Western blot assay. The C28/I2 cells were transfected with oe-vector or oe-Sox8, treated with MSU (200 μg/mL) for 24 h, and stimulated with LY294002 (10 μM) for 1 h. The expression of Beclin1 (A, B) from the indicated groups was detected by Western blot assay. The ratio of LC3-II/I (C, D) in the presence or absence of bafilomycin A1 was detected by Western blot assay. Sox8 knockdown increased the autophagy of C28/I2 cells induced by MSU. The C28/I2 cells were transfected with siRNA-NC or siSox8, then they were treated with MSU (200 μg/ml) for 24 h. The expression of Beclin1 (E, F) and ATG5 (E, G) from the indicated groups were detected by western blot assay. The ratio of LC3-II/I (H, I) in the presence or absence of bafilomycin A1 was detected by western blot assay. Results are the mean ± SD for three individual experiments. *P < 0.05, **P < 0.01.

Back to article page