Fig. 5: Relevant molecular signaling in spermatid development. | Cell Death Discovery

Fig. 5: Relevant molecular signaling in spermatid development.

From: Single-cell RNA sequencing reveals cell landscape following antimony exposure during spermatogenesis in Drosophila testes

Fig. 5

A F-actin staining for the investigation of IC structure in control and Sb testes. Representative ICs were shown with white arrows and abnormal IC structures were labelled with yellow arrows. B Immunostaining of Orb (red) mainly to label elongated spermatids in control and Sb testes. C Correlation analysis between Seminal Fluid Proteins and Mst57D signatures related in Spermatids cluster. D Correlation analysis between Mst57D and Serpin signatures in Spermatids cluster. E Correlation analysis between Serpin and Seminal Fluid Proteins signatures in Spermatids cluster. F qRT-PCR analysis of representative DEGs for Mst57D signature in testes. G qRT-PCR analysis of representative DEGs for Serpin signature in testes. H qRT-PCR analysis of representative DEGs for Seminal Fluid Proteins signature in testes. DNA was stained with Hoechst33342 (blue). **P < 0.01, ***P < 0.001, scale bar: 50 μm.

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