Fig. 1: PID1 levels predicted resistance to mitochondria-dependent apoptosis.

A Protein levels of PID1 in three human hepatoma cell lines (Hep3B, HepG2 and SK-Hep-1) and two mice hepatoma cell lines (H22, Hepa1–6). B Cell viability curves of three human hepatoma cell lines and two mice hepatoma cell lines treated with the indicated doses of H₂O₂ for 24 h. C–D Flow cytometry analysis measuring apoptosis in four hepatoma cell lines treated with H₂O₂ (200 μM) for 24 h. E–F H₂DCFDA probe was used to detect intracellular ROS in four hepatoma cell lines treated with H₂O₂ (200 μM) for 24 h by flow cytometry analysis, and mean fluorescence intensity (MFI) was calculated. G–H JC-1 probe was used to detect mitochondrial membrane potential in four hepatoma cell lines treated with H₂O₂ (200 μM) for 24 h by flow cytometry analysis, and Red/Green Ratio was calculated. Data are expressed as mean ± SD (n = 3). **p < 0.01; ***p < 0.001.