Fig. 1: TNF-α-mediated cellular events in chondrocytes. | Cell Death Discovery

Fig. 1: TNF-α-mediated cellular events in chondrocytes.

From: Inhibition of TRADD ameliorates chondrocyte necroptosis and osteoarthritis by blocking RIPK1-TAK1 pathway and restoring autophagy

Fig. 1

Chondrocytes were treated with TNF-α (5 ng/ml) for 0, 1, 3, 6, 12, and 24 h, the protein expression of iNOS, COX2, COL2A1, MMP13, P62, and LC3 was examined (A, C). Chondrocytes were pre-treated with z-VAD for 2 h and then exposed to TNF-α for 0, 0.5, 1, 3, 6, and 12 h respectively, the phosphorylation of RIPK3 and MLKL was detected using western blot (E). (B, D, F) Quantitative analysis of protein bands by image J and data in figures are expressed as means ± SD. *p < 0.05, **p < 0.01. C-caspase-3, cleaved-caspase-3. z-VAD, Z-VAD-FMK.

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