Fig. 2: ERK MAPK pathway regulates SMN protein level in MNs. | Cell Death Discovery

Fig. 2: ERK MAPK pathway regulates SMN protein level in MNs.

From: ERK MAPK signaling pathway inhibition as a potential target to prevent autophagy alterations in Spinal Muscular Atrophy motoneurons

Fig. 2: ERK MAPK pathway regulates SMN protein level in MNs.

a Six-day CD1 cultured MNs, b 6-day WT (left) and mutSMA (right) cultured MNs, and c 7-day differentiated Control (left) and SMA (right) human MNs were washed and treated with 20 μM U0126 (U0) during 24 h or left untreated. Cell lysates were then obtained and submitted to western blot analysis using anti-phospho-ERK (p-ERK) or anti-SMN antibodies. Membranes were stripped and reprobed with anti-ERK (pan-ERK) antibody or with anti-α-tubulin antibody. Graphs represent the expression of Smn vs α-tubulin or p-ERK vs pan-ERK or pan-ERK vs α-tubulin, and correspond to the quantification of at least 4 independent experiments ± SEM. Symbols indicate differences using Student t test (**p < 0.01; ****p < 0.0001) or Mann-Whitney test (#p < 0.05; ###p < 0.001).

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