Fig. 3: ERK MAPK pathway regulate SMN mRNA levels in MNs. | Cell Death Discovery

Fig. 3: ERK MAPK pathway regulate SMN mRNA levels in MNs.

From: ERK MAPK signaling pathway inhibition as a potential target to prevent autophagy alterations in Spinal Muscular Atrophy motoneurons

Fig. 3: ERK MAPK pathway regulate SMN mRNA levels in MNs.

Total RNA was extracted from 20 μM U0126-treated (+U0) or non-treated (-U0) (a) 6-day cultured CD1 MNs or (b) 7-day differentiated Control and SMA human MNs. RNA was reverse transcribed to cDNA. Smn (a) and SMN (b) were amplified through RT-qPCR. Gapdh (a) and GAPDH (b) were used as control. Graph values are the mean of Smn (a) and SMN (b) gene expression from 3 independent differentiation cycles ± SEM. Asterisks indicate significant differences using Student t test (*p < 0.05; **p < 0.01).

Back to article page