Fig. 2: SESN2 inhibits glycolysis via reduction of HK2.

A Western blot analysis of key glycolytic pathway enzymes along with LDHA and SESN2 in HepG2 cells cultured with (+) or without (−) 4 g/L glucose for 12 h. Actin served as a loading control throughout. B Glycolysis stress test profiles measuring the extracellular acidification rate (ECAR) in HepG2 cells after culture in medium with (+) or without (−) 4 g/L glucose (Glc) for 12 h. C Glucose uptake measured with 2-NBDG in the cells from B. D Western blot analysis of key glycolytic pathway enzymes along with LDHA and SESN2 in HepG2 cells after transduction with control (pLKO.1) or independent shRNAs targeting SESN2 (shSESN2#1 and #2, respectively). E, F Glycolysis stress test profiles (E) and glucose uptake (F) measured in the cells from D. F Glucose uptake of HepG2 cells transduced with control (pLKO.1) or shRNAs targeting SESN2 were measured with 2-NBDG. G Western blot analysis of key glycolytic pathway enzymes along with LDHA and SESN2 in HepG2 cells 24 hr after transfection with Flag control or Flag-SESN2 vectors. H, I Glycolysis stress test profiles (H) and glucose uptake (I) measured in the cells from G. A–I Data represent three independent experiments. Data are mean ± SD, n = 3, *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant, two-tailed paired Student’s t test.