Fig. 5: NEDD4 promoted the degradation of Runx1 by ubiquitination. | Cell Death Discovery

Fig. 5: NEDD4 promoted the degradation of Runx1 by ubiquitination.

From: GATA4-activated lncRNA MALAT1 promotes osteogenic differentiation through inhibiting NEDD4-mediated RUNX1 degradation

Fig. 5

A The knockdown efficiency of shNEDD4 was measured by qRT-PCR. B The protein levels of NEDD4 and Runx1 were detected by Western blotting after silencing NEDD4. C Co-IP used to analyze the interplay between NEDD4 and Runx1. D The turnover curve of Runx1 was measured in cells treated with cycloheximide (CHX). E Co-IP analysis of Myc-tagged Runx1 with Flag-tagged NEDD4. Mean ± SD, n = 3, *p < 0.05, **p < 0.01, ***p < 0.001. Statistical analysis was carried out by one-way ANOVA.

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