Fig. 3: Coculture of PMA-primed THP-1 cells with Huh7 cells promoted M2 polarization, macrophage CXCL1 secretion and HCC cell migration and invasions. | Cell Death Discovery

Fig. 3: Coculture of PMA-primed THP-1 cells with Huh7 cells promoted M2 polarization, macrophage CXCL1 secretion and HCC cell migration and invasions.

From: Blocking the CXCL1-CXCR2 axis enhances the effects of doxorubicin in HCC by remodelling the tumour microenvironment via the NF-κB/IL-1β/CXCL1 signalling pathway

Fig. 3

A Schematic representation of the in vitro model for the coculture of PMA-primed THP-1 cells with Huh7 cells. B CD14, CD68, and CD163 production detected in PMA-primed THP-1 cells by qRT-PCR. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01 (n = 3/group). C, D The relative mRNA expression of CD163, CD206, and CXCL1 in macrophages in the coculture system. Data are expressed as the mean ± SEM. ***P < 0.001 (n = 3/group). E CXCL1 levels in the coculture supernatant (CM) were measured by ELISA. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01 (n = 5/group). F, G Photograph and quantification of Huh7 cell migration and invasion in the coculture system at different ratios of macrophages to Huh7 cells. H, I Photograph and quantification of macrophage migration in the coculture system at different ratios of macrophages to Huh7 cells.

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