Fig. 4: Combined treatment with DOX and anti-CXCR2 affected CXCL1 expression, tumour progression, and macrophage enrichment. | Cell Death Discovery

Fig. 4: Combined treatment with DOX and anti-CXCR2 affected CXCL1 expression, tumour progression, and macrophage enrichment.

From: Blocking the CXCL1-CXCR2 axis enhances the effects of doxorubicin in HCC by remodelling the tumour microenvironment via the NF-κB/IL-1β/CXCL1 signalling pathway

Fig. 4

A–C Photographs and maximun tumour diameter measurements of mice, which were treated with anti-CXCR2 (SB225002) antibody from two days after injecting different Hepa1–6 cells are shown, n = 5. D, M The IC50 values of anti-CXCR2 and DOX were measured by MTT. Data are expressed as the mean ± SEM. (n = 5/group). E, F Photograph and quantification of HCC cell migration and invasion in the coculture system with anti-CXCR2 antibody. G qRT-PCR analyses detecting the expression level of CXCL1 in macrophages after coculture and treatment with anti-CXCR2 antibody. Data are expressed as the mean ± SEM. **P < 0.01, ***P < 0.001 (n = 3/group). H CXCL1 levels in coculture supernatant (CM) and treated with anti-CXCR2 were measured by ELISA. Data are expressed as the mean ± SEM. **P < 0.01, ns, nonsignificant (n = 5/group). I, P qRT-PCR analyses detecting the expression levels of CD163 and CD206 in macrophages after coculture and treatment with DOX and anti-CXCR2 separately or in combination. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ns, nonsignificant (n = 3/group). J–L Photographs and maximun tumour diameter measurements of mice, which were treated with DOX or combined with anti-CXCR2 (SB225002) antibody from three days after injecting different Hepa1–6 cells are shown, n = 5. N, O Photograph and quantification of HCC cell migration and invasion in the coculture system with DOX or combined with anti-CXCR2 antibody. Q–S IHC analysis of the expression of CXCL1, F4/80, and CD206 in mouse tumour tissues that received different treatments (Q, R), and the relative expression level of F4/80 or CD206 (S), Scale bar, 100 μm.

Back to article page