Fig. 1: The number of osteoclast-derived apoptotic bodies gradually decreases in the process of osteoporosis.

A Representative micro-CT images of osteoporotic mice at various stages. B Quantification of bone volume fraction and mean thickness of trabecular bone, n = 5. C Schematic representation of the apoptotic bodies extraction from femoral bone marrow. D PE-RANK and FITC-Annexin V label 1–5 μm osteoclast-derived apoptotic bodies. E Quantification of total apoptotic bodies and osteoclast-derived apoptotic bodies from femoral bone marrow in each time period, n = 4. The data in each panel represent the means ± SD. p values were obtained by student’s two-tailed unpaired t test, *p < 0.05, **p < 0.01, ns non-significant.