Fig. 1: CCA cells-secreted TGF-β1 promoted fibroblast activation. | Cell Death Discovery

Fig. 1: CCA cells-secreted TGF-β1 promoted fibroblast activation.

From: Circ_0020256 induces fibroblast activation to drive cholangiocarcinoma development via recruitment of EIF4A3 protein to stabilize KLF4 mRNA

Fig. 1

A Immunofluorescence staining of α-SMA and FAP in CAFs isolated from CCA patients. B Western blotting analysis of α-SMA, vimentin, and FAP in primary CAFs. C CCA cells were transfected with OE-NC or OE-TGF-β1 and the mRNA expression of TGF-β1 was assessed by qRT-PCR. D Enzyme linked immunosorbent assay (ELISA) was adopted to determine TGF-β1 level in the supernatant of CCA cells. CAFs were treated with CM of CCA cells. E CAFs viability was detected by CCK-8. F Scratch assay for evaluation of migration ability of CAFs. G CAFs invasion was assessed by Transwell assay. H Western blotting analysis of α-SMA, vimentin, FAP, p-Smad2, Smad2, p-Smad3, and Smad3 levels in CAFs. I The protein level of IL-6 in the supernatant of CAFs was measured by ELISA. *P < 0.05, **P < 0.01, and ***P < 0.001.

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