Fig. 5: Circ_0020256 recruited EIF4A3 protein to stabilize KLF4 mRNA. | Cell Death Discovery

Fig. 5: Circ_0020256 recruited EIF4A3 protein to stabilize KLF4 mRNA.

From: Circ_0020256 induces fibroblast activation to drive cholangiocarcinoma development via recruitment of EIF4A3 protein to stabilize KLF4 mRNA

Fig. 5

A Circular RNA Interactome database analysis of the potential binding between circ_0020256 and EIF4A3 protein. Circ_0020256 expression in CCA specimens (B) and cell lines (C) was detected by qRT-PCR. D Schematic diagram of circ_0020256 formation and Sanger sequencing was used for identifying circ_0020256. The expression of circ_0020256 and linear NSMCE4A mRNA after exposure to RNase R (E) or actinomycin D (F) were assessed by qRT-PCR. The interaction between circ_0020256 and EIF4A3 protein was verified by RIP (G) and RNA pull-down assay (H). I qRT-PCR analysis of circ_0020256 level in CCA cells after transfection with OE-circ_0020256 or sh-circ_0020256. J EIF4A3 expression in CCA cells with different treatments was detected by western blotting. K The positive correlation between circ_0020256 and EIF4A3 expression in CCA samples. L The stability of KLF4 mRNA after exposure to actinomycin D was evaluated by qRT-PCR. M The interaction between KLF4 mRNA and EIF4A3 protein in circ_0020256-silenced CCA cells was determined by RIP assay. 45 paired patient samples were detected in B and K. *P < 0.05, **P < 0.01, and ***P < 0.001.

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