Fig. 7: CAFs-derived IL-6 facilitated growth and migration of CCA cells via autophagy inhibition. | Cell Death Discovery

Fig. 7: CAFs-derived IL-6 facilitated growth and migration of CCA cells via autophagy inhibition.

From: Circ_0020256 induces fibroblast activation to drive cholangiocarcinoma development via recruitment of EIF4A3 protein to stabilize KLF4 mRNA

Fig. 7: CAFs-derived IL-6 facilitated growth and migration of CCA cells via autophagy inhibition.

CCA cells were treated with CAFs-derived CM or IL-6. CCA cell proliferation was evaluated by CCK-8 (A) and colony formation assay (B). C CCA cell migration was detected by scratch assay. D Transwell assay determined CCA cell invasion. E Western blotting analysis of E-cadherin, N-cadherin, slug, MMP-2 and fibronectin protein levels in CCA cells. F The images of immunofluorescence staining of LC3. G Western blotting analysis of LC3 II/I, p62, beclin-1 protein abundance. *P < 0.05, **P < 0.01, and ***P < 0.001.

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