Fig. 6: The knockdown of norepinephrine transporter (NET) antagonizes NE-promoted subcutaneous CT26 tumors’ formation in vivo with corresponding changes of protein phosphatase 2 scaffold subunit alpha (PPP2R1A), Akt activation, VEGF, and CD34 in tumor xenografts. | Cell Death Discovery

Fig. 6: The knockdown of norepinephrine transporter (NET) antagonizes NE-promoted subcutaneous CT26 tumors’ formation in vivo with corresponding changes of protein phosphatase 2 scaffold subunit alpha (PPP2R1A), Akt activation, VEGF, and CD34 in tumor xenografts.

From: Venlafaxine antagonizes the noradrenaline-promoted colon cancer progression by inhibiting the norepinephrine transporter

Fig. 6: The knockdown of norepinephrine transporter (NET) antagonizes NE-promoted subcutaneous CT26 tumors’ formation in vivo with corresponding changes of protein phosphatase 2 scaffold subunit alpha (PPP2R1A), Akt activation, VEGF, and CD34 in tumor xenografts.

A Following acclimatization, male BALB/c mice were randomly divided into four groups (n = 5): CT26-Lv-shCtrl.+saline, CT26-Lv-shNET-3+saline, CT26-Lv-shCtrl.+NE, and CT26-Lv-shNET-3+NE. Male BALB/c mice were intraperitoneally administered with saline or NE (0.125 mg/kg) daily. One week later, 1 × 106 stable cells (CT26-Lv-shNET-3 or CT26-Lv-shCtrl.) were injected subcutaneously into the mice separately. In addition, mice of each group were treated with saline or NE for 17 days. Volumes of tumor xenografts were measured (upper left). On day 17 following cell inoculation, mice were scarified, and tumor xenografts were removed. The gross morphology of tumors, tumor volume, and tumor weight of each group were detected (upper right, lower left, and lower right). B Hematoxylin and eosin (HE) staining of the xenograft sections and CD34 immunohistochemical analysis was performed. C Xenograft lysates were collected, and the expression levels of PPP2R1A, pAkt, Akt, VEGF, and CD34 were analyzed by western blotting. The band intensities of each protein were normalized by that of β-actin and the average value was obtained from the repetition in each group. Data are expressed as mean ± SD, *P < 0.05, **P < 0.01, or ***P < 0.001 in A and C.

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