Fig. 3: The effect of BCar on the viability of human normal mammary epithelial cells (HME) and breast cancer cells. | Cell Death Discovery

Fig. 3: The effect of BCar on the viability of human normal mammary epithelial cells (HME) and breast cancer cells.

From: Benzimidazole carbamate induces cytotoxicity in breast cancer cells via two distinct cell death mechanisms

Fig. 3

Log-phase growing HME cells were incubated with BCar for 48 h at the indicated concentrations, washed, and incubated in a growth medium for 14 days. The surviving cells were visualized by crystal violet staining. A Representative samples from cell viability assay. B Cell survival in the resulting samples was quantified using ImageJ and the results are shown as mean ± s.d. of two sets of experiments in duplicate samples. EC50 values were determined using nonlinear regression with GraphPad Prism software. C Cells were photographed using phase-contrast optics. Scale bars represent 100 µm. D HME and breast cancer cells were incubated with 1 µM BCar for the specified time. Dead cells were quantified by the trypan blue exclusion assay. Results are shown as mean ± s.d. of two sets of experiments in duplicate samples.

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