Fig. 5: BCar-induced accumulation of multiple micronuclei, a hallmark of mitotic catastrophe, in p53-deficient breast cancer cells but not in p53-proficient normal HME or breast cancer cells. | Cell Death Discovery

Fig. 5: BCar-induced accumulation of multiple micronuclei, a hallmark of mitotic catastrophe, in p53-deficient breast cancer cells but not in p53-proficient normal HME or breast cancer cells.

From: Benzimidazole carbamate induces cytotoxicity in breast cancer cells via two distinct cell death mechanisms

Fig. 5

A Cells were incubated with increasing concentrations of BCar for 48 h and immunoblotted for the levels of Histone H3-S10 phosphorylation (a hallmark of mitotic cells), Cdc2-T14/Y15-phosphorylation (a hallmark of G2 checkpoint activation), and p21 (p53 target gene). GAPDH serves as a protein loading control. Note: Fig. 5A (lower panels): Histone H3 and GAPDH panels were from the same gels with different exposures. B Cells were incubated with 1 µM BCar for the times indicated, stained microtubules with an anti-α/β tubulin antibody, and nuclei with DAPI. The structure of microtubules and integrity of the nuclei were analyzed using a Zeiss-810 confocal laser-scanning microscope, as described in Materials and Methods. Scale bars, 10 μm.

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