Fig. 6: The effect of BCar on apoptosis, autophagy, and senescence in human normal mammary epithelial cells and breast cancer cells. | Cell Death Discovery

Fig. 6: The effect of BCar on apoptosis, autophagy, and senescence in human normal mammary epithelial cells and breast cancer cells.

From: Benzimidazole carbamate induces cytotoxicity in breast cancer cells via two distinct cell death mechanisms

Fig. 6

A, B Log-phase growing cells were incubated with 1 µM BCar as indicated and analyzed for the integrity of PARP and caspase 3 cleavage (hallmarks of apoptosis), the levels of LC3A/B-I/II (a hallmark of autophagy) and GAPDH by Western blot analysis. C Cells were incubated with 1 µM BCar for 48 h, washed with plain medium, and then incubated for 1 week in regular growth medium. The resulting cells were analyzed for senescent cells with SA-β-gal assay. Blue-color-stained senescent cells were visualized by IMT-2 Olympus phase contrast microscope, quantified by ImageJ software and the percentage of senescent cells was analyzed by SigmaPlot software. The study was repeated twice in duplicates with similar results obtained. Arrow points at a senescent cell that is positive in β-galactosidase staining. Scale bars, 100 μm.

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