Fig. 7: Knockdown of p53 and BCar treatment trigger the induction of mitotic catastrophe in MDA-MB-175-VII breast cancer cells, which is accompanied by the suppression of apoptosis induction. | Cell Death Discovery

Fig. 7: Knockdown of p53 and BCar treatment trigger the induction of mitotic catastrophe in MDA-MB-175-VII breast cancer cells, which is accompanied by the suppression of apoptosis induction.

From: Benzimidazole carbamate induces cytotoxicity in breast cancer cells via two distinct cell death mechanisms

Fig. 7

Cells were transfected with p53-siRNA for 48 h and then incubated with 1 µM BCar for 48 h. For the day 3 time point, cells were washed with plain medium after BCar treatment and incubated in growth medium for an additional 24 h before analysis. A Cell lysates were analyzed for p53 protein levels by Western blot. B Microtubule structures were visualized by immunostaining with an anti-α/β tubulin antibody. The nuclei were stained with DAPI and analyzed for their integrity by fluorescent confocal microscopy as described in Fig. 5B. Scale bar = 10 µm. Bar graph, the indicated cells were quantified for the percentage of cells containing multi-nuclei and the results are shown as mean ± s.d. of seven samples. C. Cell lysates were analyzed for the integrity of PARP protein and GAPDH by Western blot analysis.

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