Fig. 2: IGF2BP3 promotes proliferation of postnatal CMs in vitro. | Cell Death Discovery

Fig. 2: IGF2BP3 promotes proliferation of postnatal CMs in vitro.

From: IGF2BP3 promotes adult myocardial regeneration by stabilizing MMP3 mRNA through interaction with m6A modification

Fig. 2: IGF2BP3 promotes proliferation of postnatal CMs in vitro.

A Flow cytometry analysis shows the increase of pH3-positive P7 CMs overexpressing IGF2BP3. B Western blot analysis of pH3 and Aurora B proteins in P7 CMs overexpressing IGF2BP3. C Effects of IGF2BP3 overexpression on CM proliferation. Representative images and statistical analyses of P7 CMs stained by cell proliferation markers. For each condition, 500–600 cells were analyzed from 6 independent samples (*P < 0.05). Scale bar: 50 µm. D Flow cytometry analysis shows increased proportion of CMs overexpressing IGF2BP3 at the S and G2/M phases of the cell cycle. E Effects of IGF2BP3 knockdown on CM proliferation. Representative images and statistical analyses of P1 CMs stained by indicated cell proliferation markers. For each condition, 500–600 cells were analyzed from 8 independent samples (*, P < 0.05). Scale bar: 50 µm. F Flow cytometry analysis shows decreased proportion of P1 CMs at the S and G2/M phases of the cell cycle following IGF2BP3 knockdown. G–I TUNEL assay shows reduced apoptosis following overexpression of IGF2BP3 in MI heart. Data are the mean ± s.e.m. from three independent experiments (**P < 0.01). Scale bar: 50 µm.

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