Fig. 3: Cyclic mechanical stretch increased RhoA activation, F-actin polymerization and Sun2 expression in Z24−/− MSCs.
From: Nuclear softening mediated by Sun2 suppression delays mechanical stress-induced cellular senescence
A Z24−/− MSCs were loaded for cyclic mechanical stretch with a FlexCell cell stretching bioreactor (10% uniaxial cyclic stretch at 0.5 Hz of frequency for 24 h). Immunofluorescent staining of RhoA GTPase (green) and staining of F-actin with Alex Fluo 594 phalloidin (red) showed increased level of RhoA protein and F-actin polymerization Z24−/− MSCs after stretch. B Statistics of the level of RhoA protein in Z24−/− MSCs with and without stretch. C Immunofluorescent staining of Sun2 (red) showed increased Sun2 expression in WT and Z24−/− MSCs after stretch. Arrows mark nucleus with high Sun2 expression or nuclear blebs. D Statistics of the level of Sun2 protein in WT and Z24−/− MSC with or without stretch. N ≥ 6 for data analysis, with at least three biological replicates and two technical replicates. * indicates p < 0.05.
