Fig. 6: Suppression of Sun2 expression in Z24−/− MSCs lowered nuclear stiffness promoted by cyclic mechanical stretch.
From: Nuclear softening mediated by Sun2 suppression delays mechanical stress-induced cellular senescence
A Testing the nuclear stiffness of Z24−/− MSCs by atomic force microscope (AFM) loaded with a Bruker AFM probe. B Z24−/− MSCs were transfected with Sun2 siRNA or control siRNA for 36 h, and then loaded for cyclic mechanical stretch (10% uniaxial cyclic stretch at 0.5 Hz of frequency for 24 h). Mechanical stiffness at the nucleus was then detected with AFM probe (arrows). Sun2 staining of the cells was performed to localize and identify the nucleus of Z24−/− MSCs in different groups. C Mechanical force detected by NanoScope analysis when AFM probe approach and retract from cell nucleus, reflecting the stiffness of nuclear matrix AFM probe traveled through. D The nuclear stiffness (kPa) calculated by Young Modulus force. N ≥ 6 for data analysis, with at least three biological replicates and two technical replicates. * indicates p < 0.05.
