Fig. 1: Sensitivity of alternatively activated macrophages to lipid peroxidation-driven ferroptosis.
From: Role of liensinine in sensitivity of activated macrophages to ferroptosis and in acute liver injury
a–e CCK-8 assay for cell viability after the treatment of macrophages (M0, M1, AAM) with the apoptosis inducer (20 μM, 24 h), pyroptosis inducers (7 μg/ml, 24 h), necrosis inducer (4×, 24 h), RSL3 inducer (5 μM, 5 h), and erastin inducer (60 μM, 24 h). f–i AAM was treated with RSL3 (5 μM, 5 h) in the presence or absence of Fer-1 (400 nM). f PI staining to assess cell death, Scale bar = 200 µm. g Live cell fluorescence imaging to detect lipid peroxide production using Liperfloo, Scale bar = 100 µm. h Live cell fluorescence imaging of FerroOrange (red), Scale bar = 100 µm. i Fluorescence imaging of the superoxide anion fluorescence detection probe Dihydroethidium (DHE), Scale bar = 100 µm. j Expression of the 4-hydroxynonenal (4-HNE) protein was measured by immunofluorescence, Scale bar = 50 µm. Results were presented as mean ± SD. (n = 3, ****p < 0.0001. Con control, NS not significant).
