Fig. 4: The ferroptosis induced by mechanical stress shows more obvious in PA-treated C2C12. | Cell Death Discovery

Fig. 4: The ferroptosis induced by mechanical stress shows more obvious in PA-treated C2C12.

From: Ferrostatin-1 alleviates the damage of C2C12 myoblast and mouse pelvic floor muscle induced by mechanical trauma

Fig. 4

A Cell viability was measured after cells treated with 0, 50, 75, 100, 125 μM PA. B The effect of PA for cell lipid accumulation was validated by cellular TG determination and Oil Red O staining. C–E Lipid peroxidation of cells was determined with MDA Assay Kit, ROS was analyzed by DCFH-DA with flow cytometry, and total cellular iron was detected with Iron Assay Kit after C2C12 CMS for 4 h, which was cultured with 100 μM PA for 48 h. F the living/dead cells were indicated by Calcein AM/PI staining (Calcein AM, living cells, green; PI, dead cells, red). G The representative proteins of ferroptosis were analyzed by western blotting. CON, control, CMS cyclic mechanical strain, PA palmitic acid. A, B (left), C, D (right), F (down), G (right) Data are expressed as the mean ± SEM. NS, no significance, *P < 0.01, **P < 0.05, ***P < 0.001 (n = 3/group).

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