Fig. 3: Notch1 upregulates CD73 transcription by binding to its promoter. | Cell Death Discovery

Fig. 3: Notch1 upregulates CD73 transcription by binding to its promoter.

From: Notch1 promotes resistance to cisplatin by up-regulating Ecto-5′-nucleotidase (CD73) in triple-negative breast cancer cells

Fig. 3: Notch1 upregulates CD73 transcription by binding to its promoter.

A Graphic representation of the Notch CSL binding site region in the CD73 proximal promoter and Negative control region. B The recruitment of Notch1 to the CD73 promoter in MDA-MB-231 cells was assessed using ChIP assays. PCR products were detected in the presence of anti-Notch1 primary antibody. C Schematic illustration of the establishment of CD73 promoter luciferase reporter (pGL3-CD73pro-WT) and individual Notch CSL binding site deletion mutant (pGL3-CD73pro-MT) vector. D Dual-Luciferase assays were performed in MDA-MB-231 cell by co-transfection with LV201-SH, LV201-N1ICD, pGL3-CD73pro-WT or pGL3-CD73pro-MT and luciferase activity was normalized to the Renilla minimal. E Notch1 was silenced in MDA-MB-231 cells by siRNA and then co-transfected with the pGL3-CD73pro-WT or pGL3-CD73pro-MT. Dual-Luciferase values were used to indicate promoter activity. *p < 0.05, **p < 0.01, and ***p < 0.001 (Student’s t test) as compared with control cells. Data were presented as the mean ± SEM. (n = 3).

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